Stability and Transepithelial Transport of Oligopeptide (KRQKYD) with Hepatocyte-protective Activity from Jinhua Ham in Human Intestinal Caco-2 Monolayer Cells

The study evaluated the stability of an oligopeptide (KRQKYD) and its transport mechanism by simulating gastrointestinal digestion a model human intestinal Caco-2 monolayer cells in vitro. In this study, effects environmental factors (temperature, pH NaCl concentration) simulated on KRQKYD were indicators levels alanine transaminase (ALT), aspartate (AST) malondialdehyde (MDA) alcohol-induced hepatocyte injury model. results showed that still maintained satisfactory hepatocyte-protective activity after treatment with different temperatures (20-80 ℃), (3-9), concentration (1-7%) digestion, which indicated good to digestion. Furthermore, intact could be absorbed Papp value (9.70 ± 0.53) ×10−7 cm/s. Pretreatment energy inhibitor (sodium azide), competitive peptide transporter (Gly-Pro) transcytosis wortmannin did not decrease level transepithelial transport, indicating was associated dependent, vector mediated endocytosis. tight junction disruptor cytochalasin D significantly increased (P < 0.05), suggesting paracellular transport.